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KMID : 0043319860090010045
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Archives of Pharmacal Research
1986 Volume.9 No. 1 p.45 ~ p.47
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Hydrolysis of the Nitrile group in alpha-Aminophenylacetonitrile by Nitrilase; Development of a New Biotechnology for Stereospecific Production of S-alpha-Phenylglycine
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Choi SY
Goo YM
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Abstract
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Phenylglycine was obtained as the sole metabolite when alpha-aminophenylacetonitrile was fed to the culture broth of Aspergillus furmigatus. The isolated phenylglycine showed L-configuration with 80% optical purity. Examination of the hydrolysis of the substrate to phenylglycine with cell free extracts, and the supernatant fraction and the particulate fraction both of which were obtained after ultracentrifugation of the cell free extract at 100,000g, indicated that the nitrile group hydrolyzing enzymes, nitrilase existed not only in cytoplasm, but in microsome fractions.
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